منابع مشابه
Multiparameter Flow Cytometry: Advances in High Resolution Analysis
Over the past 40 years, flow cytometry has emerged as a leading, application-rich technology that supports high-resolution characterization of individual cells which function in complex cellular networks such as the immune system. This brief overview highlights advances in multiparameter flow cytometric technologies and reagent applications for characterization and functional analysis of cells ...
متن کاملSingle particle high resolution spectral analysis flow cytometry.
BACKGROUND While conventional multiparameter flow cytometers have proven highly successful, there are several types of analytical measurements that would benefit from a more comprehensive and flexible approach to spectral analysis including, but certainly not limited to spectral deconvolution of overlapping emission spectra, fluorescence resonance energy transfer measurements, metachromic dye a...
متن کاملAutomation in high-content flow cytometry screening.
High-content flow cytometric screening (FC-HCS) is a 21st Century technology that combines robotic fluid handling, flow cytometric instrumentation, and bioinformatics software, so that relatively large numbers of flow cytometric samples can be processed and analysed in a short period of time. We revisit a recent application of FC-HCS to the problem of cellular signature definition for acute gra...
متن کاملQuantitative karyotyping of human chromosomes by dual beam flow cytometry.
Dual beam flow cytometry of chromosomes stained with Hoechst 33258 and chromomycin A3 has been proposed as a method for quantitative classification of human chromosomes (bivariate flow karyotyping). In this paper we investigate the sources and magnitudes of variability in the mean fluorescence intensities of each chromosome group resolved in bivariate flow karyotypes and study the impact of thi...
متن کاملIdentification and characterization of plasma cells in normal human bone marrow by high-resolution flow cytometry.
The low frequency of plasma cells and the lack of specific cell surface markers has been a major obstacle for a detailed characterization of plasma cells in normal human bone marrow. Multiparameter flow cytometry enabled the identification of plasma cells in normal bone marrow aspirates. The plasma cells were located in a unique position in the correlation of forward light scattering, orthogona...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1978
ISSN: 0022-1554,1551-5044
DOI: 10.1177/26.8.357646